Liqui-CultTM test kits are used to detect microbial growth in fluids, in the laboratory and in the workplace. Areas of application include metalworking fluids, circulating cooling and process water, hydrocarbons (diesel fuel), and other liquids such as cleaners, cosmetics, liquid soaps, detergents, etc.
Liqui-CultTM testing determines the type and degree of infestation usually measured by the more expensive plate count system. To use, simply fill the 5cc syringe (included in your kit) with the fluid to be tested. Inject the complete 5cc's through the rubber cap of the test bottle containing the liquid agar and shake it vigorously. Incubate for 30 hours for bacterial growth or 72 hours for fungal growth and compare it to the appropriate photo charts to determine the degree of contamination.
Directions:
Capture a fuel sample in a clean dry container.
Remove center seal from metal cap of test bottle. Unwrap syringe. remove needle guard and insert tip into liquid to be tested. Draw plunger back and fill the syringe with complete 5cc's fo fluid to be tested.
Inject needle through rubber cap on bottle and push down on syringe plunger, emptying contents completely into it. Remove syringe from bottle, replacing needle guard and discard according to local, state and federal guidelines.
Incubate bottle at rooem temperature for 30 hours for microbial growth and 72 hours in total for fungal growth.
Bacterial Growth: After 30 hours compare the color of sample bottle to the color chart.
Fungal Growth: After 7 hours compare the color of the test bottle to the color chart.
Storage and Disposal: Liqui-Cult should be stored at room temperature (68-75 deg. F). Protect from light. Need not be refrigerated. Under a fume hood fill the bottle with bleach . . . let stand 24 hours.
Note that in performing testing where a bio-cide or other similar product has been used in fuel, the test will likely result in a negative reading for microbial and fungal contamination, but fuel degradation and contamination from other sources is common and the test will not disclose other problems.
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